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automatic counting of spontaneous calcium activity transient peak/min or sec

Mon, 10/18/2010 - 10:31 am

Dear Friends,

I am rather new to the use of Igor, so this may be a very nave question. I would appreciate advice on creating an efficient procedure for implementing it.

I am recording spontaneous calcium activity and I would like to count spontaneous calcium activity transient peak/min or sec. I would like to count it automatically like mini analysis did for patch clamp data. Here I would like to count it for several cells traces at a time and I was wondering if there is procedure to do it using Igor.

Many thanks for your help,
Manoj

johnweeks

You might want to look into the Neuromatic package, written by an Igor user who is a neuroscientist. Find it at http://www.NeuroMatic.ThinkRandom.com/. It has an optional module for doing data acquisition using either National Instruments or Instrutech (now HEKA) DAQ hardware.

John Weeks
WaveMetrics, Inc.
support@wavemetrics.com

October 18, 2010 at 11:20 am - Permalink

RGerkin

Neuromatic can do this (it does mini detection, which is basically the same thing). I also have a package for event detection like this, although a few changes might be in order for slow events like calcium transients. You can direct message me if you're interested.

October 25, 2010 at 02:44 am - Permalink