Need program used for peak deconvolution

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Mon, 10/03/2016 - 07:52 am

Do anyone knows how to use Igor Pro for peak deconvolution and has a program that can be used in the software? Please share the program with me, thanks very much!

The detail problem is that I want to deconvolute the fluorescence decay profiles, which consists the instrument response function and the fluorescence intensity from the sample. Do anyone have experiences on this stuff? Thanks!
Many years ago, I wrote a deconvolution routine based on an iterative method. I mention it on this thread.

http://www.igorexchange.com/node/1371

Other than this, I am unaware of any other deconvolution packages for Igor Pro.

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J. J. Weimer
Chemistry / Chemical & Materials Engineering, UAH

October 3, 2016 at 09:41 am - Permalink

Perhaps our Multipeak Fit 2 package will do what you need. To learn more about it, select File->Example Experiments->Curve Fitting->Multi-peak fit 2 Demo.

Quote:

The detail problem is that I want to deconvolute the fluorescence decay profiles, which consists the instrument response function and the fluorescence intensity from the sample. Do anyone have experiences on this stuff? Thanks!

Ah- you are talking about a single peak? No doubt it is a convolution of some sort of impulse-like shape with an exponential decay. It's possible that Multipeak Fit 2 can still help you, even though it's only a single peak. I would look at the ExpModGauss and the ExpConvExp peak shapes.

John Weeks
WaveMetrics, Inc.
support@wavemetrics.com

October 3, 2016 at 09:43 am - Permalink

jjweimer wrote:
Many years ago, I wrote a deconvolution routine based on an iterative method. I mention it on this thread.

http://www.igorexchange.com/node/1371

Other than this, I am unaware of any other deconvolution packages for Igor Pro.

--
J. J. Weimer
Chemistry / Chemical & Materials Engineering, UAH


Thanks for the information, but I didn't find where to download the procedure in your link, can you send me the download link?

October 5, 2016 at 11:46 am - Permalink

johnweeks wrote:
Perhaps our Multipeak Fit 2 package will do what you need. To learn more about it, select File->Example Experiments->Curve Fitting->Multi-peak fit 2 Demo.

Quote:

The detail problem is that I want to deconvolute the fluorescence decay profiles, which consists the instrument response function and the fluorescence intensity from the sample. Do anyone have experiences on this stuff? Thanks!

Ah- you are talking about a single peak? No doubt it is a convolution of some sort of impulse-like shape with an exponential decay. It's possible that Multipeak Fit 2 can still help you, even though it's only a single peak. I would look at the ExpModGauss and the ExpConvExp peak shapes.

John Weeks
WaveMetrics, Inc.
support@wavemetrics.com


Thanks very much, I will try this soon!

October 5, 2016 at 11:47 am - Permalink

sli2008 wrote:
...
Thanks for the information, but I didn't find where to download the procedure in your link, can you send me the download link?


The method that @johnweeks mentions should do better than what I have. AFAIR, I never did generate a revised version of the deconvolution routine, and it is better suited to deconvolve a transmission function from a peak in spectroscopy than an exponential decay curve.

--
J. J. Weimer
Chemistry / Chemical & Materials Engineering, UAH

October 5, 2016 at 05:18 pm - Permalink

You may also want to check out the Spectral Deconvolution Demo experiment under File Menu->Example Experiments->Analysis

A.G.
WaveMetrics, Inc.

October 11, 2016 at 03:08 pm - Permalink