Image Acquisition Example
The program is designed for coordinating image acquisition using the Qimaging XOP (WaveMetrics) and Qimaging Retiga Exi Fast 1394 monochrome cooled CCD camera from Qimaging with a laser combiner (for a HeCd and Ar ion laser providing laser lines of 442, 488, and 514 nm) manufactured by Prairie Technologies (www.prairie-technologies.com).
The laser intenstiy and "shuttering" are controlled through a NEOS acoustic optical tunable filter using an Instrutech ITC-18 data acquisition board. The ITC-18 is controlled by the ITC18 XOP furnished by Instrutech, now downloadable from HEKA.
The eventual goal is to add patch-clamp electronphysiological data acquisition to the program using the ITC-18. The setup is designed to acquire fluorescence resonsance energy transfer (FRET) imaging data using Total Internal Reflectance Fluorescence (TIRF) imaging modality on an Olympus IX71 inverted microscope equipped with a 60x 1.45 na objective.
Stephen R. Ikeda, M.D., Ph.D.
Chief, Lab. of Molecular Physiology
National Institutes of Health
National Institute on Alcohol Abuse and Alcoholism
Igor Pro 8
Igor XOP Toolkit
Igor NIDAQ Tools MX